A/B testing
Also: split testing, A/B test, subject line testing
A/B testing is sending two versions of an email to small parts of your list to see which does better, then sending the winner to everyone else. Subject lines are the most common thing to test.
The most common mistake is judging the test on too few people. If each version goes to a few hundred subscribers and one gets a couple more clicks, that's probably noise, not a winner. Your ESP may show a "winner" anyway, so be skeptical of small gaps on small samples.
The second issue is what you measure. Subject line tests used to be judged on opens, but Apple Mail Privacy Protection makes opens unreliable. Judge on clicks, or on whatever you actually care about, even if it means waiting longer for results.
Test one thing at a time, so you know what made the difference. And keep a record of what you've learned. A dozen small tests on the same list, written down, are worth far more than any single result.